Eukaryotic expression, Co-IP and MS identify BMPR-1B protein-protein interaction network

BACKGROUND: BMPR-1B is part of the transforming growth factor ß super family and plays a pivotal role in ewe litter size. Functional loss of exon-8 mutations in the BMPR-1B gene (namely the FecB gene) can increase both the ewe ovulation rate and litter size. RESULTS: This study constructed a eukaryotic expression system, prepared a monoclonal antibody, and characterized BMPR-1B/FecB protein-protein interactions (PPIs). Using Co-immunoprecipitation coupled to mass spectrometry (Co-IP/MS), 23 proteins were identified that specifically interact with FecB in ovary extracts of ewes. Bioinformatics analysis of selected PPIs demonstrated that FecB associated with several other BMPs, primarily via signal transduction in the ovary. FecB and its associated interaction proteins enriched the reproduction process via BMP2 and BMP4 pathways. Signal transduction was identified via Smads proteins and TGF-beta signaling pathway by analyzing the biological processes and pathways. Moreover, other target proteins (GDF5, GDF9, RhoD, and HSP 10) that interact with FecB and that are related to ovulation and litter size in ewes were identified. CONCLUSIONS: In summary, this research identified a novel pathway and insight to explore the PPi network of BMPR-1B.

Polymorphism of bone morphogenetic protein receptor-IB [BMPR-IB] gene with litter size and kids growth of some goat breeds in Egypt

Polymerase Chain Reaction-Restriction Fragment Length Polymorphisms [PCR-RFLP] and Single Nucleotide polymorphism [SNP] techniques were used to study the association between bone morphogenetic protein receptor IB [BMPR IB] gene polymorphism with litter size trait and kids growth. Forty four Female goats were precisely selected according to their litter size and kids growth. PCR amplification of 190 bp of the BMPR-IB gene was genotyped in all goats and sequenced only in those produced the highest and lowest litter size and kids growth. Restriction analysis of PCR-RFLP using Ava II and Hind III of the BMPRIB gene [190-bp] do not produce restriction fragments. By DNA sequencing, eight single nucleotide polymorphisms [SNP's] at seven different positions were obtained. Furthermore, with translation of SNPs to corresponding amino acids, change of six amino acids in three female goats were obtained as the following, Baladi goat with high litter size, glutamic acid [E] changed to aspartic acid [P] and isoleucine [I] changed to valine [V]. In high litter size, Zaraibi goat, valine [V] changed to leucine acid [L] and glutamine [Q] changed to histidine [H] and threonine [T] changed to proline [P]. These findings can be used in a marker-assisted selection [MAS] for selection for high litter size trait in goats. There are negative relationships in most goats between SNPs in BMPR IB gene and relative growth gain [RGG]